According to the different bottom of the ELISA plate, it can be divided into flat bottom, U-shaped bottom, V-shaped bottom . 1. According to the difference of the bottom of the ELISA 96 well plate, it can be divided into flat bottom, U-shaped bottom and V-shaped bottom. The flat bottom has a low refractive index and is suitable for detection in a microplate reader The ELISA plate with U-shaped bottom has a high refractive index, which is convenient for operations such as sample addition, aspiration, and mixing. It can be directly observed by visual inspection of the color change without placing it on the microplate reader, so as to determine whether there is a corresponding immune reaction. V-bottomed microtiter plate can accurately aspirate samples